Sample ID Sample Title Source Type Cell Type Organ Type Organism Strain Age Sex Developmental Stage Disease Description RSM00646 STAT3/K5 no.29 sup-day2 cultured CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J 5 months female adult Dermatitis nothing RSM00647 B6 WT day2-sup cultured CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J 6 weeks female adult healthy nothing RSM00648 STAT3/K5 no.29 serum tissue tissue blood serum Mus musculus C57BL/6J 5 months female adult Dermatitis nothing RSM00649 B6 WT serum tissue tissue blood serum Mus musculus C57BL/6J 6 weeks female adult healthy nothing RSM00683 1 tissue n/a blood serum Mus musculus hybrid 7 weeks male adult healthy Sunflower seed oil (i.p.) Day4 3days RSM00684 2 tissue n/a blood serum Mus musculus hybrid 7 weeks female adult healthy Sunflower seed oil (i.p.) Day4 3days RSM00685 3 tissue n/a blood serum Mus musculus hybrid 7 weeks male adult healthy Tamoxifen (i.p.) Day4(age) 3days RSM00686 4 tissue n/a blood serum Mus musculus hybrid 7 weeks female adult healthy Tamoxifen (i.p.) Day4(age) 3days RSM00687 5 tissue n/a blood serum Mus musculus hybrid 9 weeks male adult Monarticular juvenile rheumatoid arthritis Sunfiower seed oil (i.p.) collagen antibody (condrex inc.) (i.p.) 10mg/animal at 7 weeks (age) RSM00688 6 tissue n/a blood serum Mus musculus hybrid 9 weeks female adult Monarticular juvenile rheumatoid arthritis Sunfiower seed oil (i.p.) Day3 (age) 4days collagen antibody (condrex inc.) (i.p.) 10mg/animal at 7 weeks (age) RSM00689 7 tissue n/a blood serum Mus musculus hybrid 9 weeks male adult Monarticular juvenile rheumatoid arthritis Tamoxifen (i.p.) 5mg/kg Day4 (age) 3days collagen antibody (condrex inc.) (i.p.) 10mg/animal at 7 weeks (age) RSM00690 8 tissue n/a blood serum Mus musculus hybrid 9 weeks female adult Monarticular juvenile rheumatoid arthritis Tamoxifen (i.p.) 5mg/kg Day4 (age) 3days collagen antibody (condrex inc.) (i.p.) 10mg/animal at 7 weeks (age) RSM00793 OT2 Th2 #1 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a male adult healthy loh15 and APC(whole spleen) stim 24h RSM00794 OT2 Th2 #2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a male adult healthy loh15 and APC(whole spleen) stim 24h RSM00795 OT2/CNS2KO Th2 #1 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a female adult healthy loh15 and APC(whole spleen) stim 24h RSM00796 OT2/CNS2KO Th2 #2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a female adult healthy loh15 and APC(whole spleen) stim 24h RSM00802 B6 Th1 R2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th1 R2 RSM00803 CNS2KO Th1 R2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th1 R2 RSM00804 B6 Th1 R3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th1 R3 RSM00805 CNS2KO Th1 R3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th1 R3 RSM00806 B6 Th2 R2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th2 R2 RSM00807 CNS2KO Th2 R2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th2 R2 RSM00808 B6 Th2 R3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th2 R3 RSM00809 CNS2KO Th2 R3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th2 R3 RSM00833 OT2 Th2 #1 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a male adult healthy loh15 and APC(whole spleen) stim 24h RSM00834 OT2 Th2 #2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a male adult healthy loh15 and APC(whole spleen) stim 24h RSM00835 OT2/CNS2KO Th2 #1 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a female adult healthy loh15 and APC(whole spleen) stim 24h RSM00836 OT2/CNS2KO Th2 #2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a female adult healthy loh15 and APC(whole spleen) stim 24h RSM00872 DO11.10 Th1 cell cultured T-helper 1 cell spleen Mus musculus BALB/c 6 weeks female adult healthy CD4T cells were enriched with MACS from spleen of DO11.10 Tg mice on a Rag2-deficient background and were stimulated stimulated with OVA peptide(Loh15) in the presece of irradiated splenocytes. Cells were cultured in the presence of mrIL-12 and IL-4 mAb for 7 days. Cells were stimulated with anti-TCRbeta mAb for 24hrs. RSM00873 DO11.10 Th2 cell cultured T-helper 2 cell spleen Mus musculus BALB/c 6 weeks female adult healthy CD4T cells were enriched with MACS from spleen of DO11.10 Tg mice on a Rag2-deficient background and were stimulated stimulated with OVA peptide(Loh15) in the presece of irradiated splenocytes. Cells were cultured in the presence of mrIL-4 and IFNg mAb for 7 days. Cells were stimulated with anti-TCRbeta mAb for 24hrs. RSM00874 DO11.10 iTreg cultured T-helper cell spleen Mus musculus BALB/c 6 weeks female adult healthy CD4T cells were enriched with MACS from spleen of DO11.10 Tg mice on a Rag2-deficient background and were stimulated stimulated with OVA peptide(Loh15) in the presece of irradiated splenocytes. Cells were cultured in the presence of rTGFbeta ,IL-4 mAb and IFNg mAb for 7 days. Cells were stimulated with anti-TCRbeta mAb for 24hrs. RSM00875 DO11.10 Th17 cell cultured T-helper cell spleen Mus musculus BALB/c 6 weeks female adult healthy CD4T cells were enriched with MACS from spleen of DO11.10 Tg mice on a Rag2-deficient background and were stimulated stimulated with OVA peptide(Loh15) in the presece of irradiated splenocytes. Cells were cultured in the presence of rTGFbeta rmIL-6, IL-4 mAb and IFNg mAb for 7 days. Cells were stimulated with anti-TCRbeta mAb for 24hrs. RSM00876 DO11.10 Tfh cell cultured T-helper cell spleen Mus musculus BALB/c 6 weeks female adult healthy CD4T cells were enriched with MACS from spleen of DO11.10 Tg mice on a Rag2-deficient background and were stimulated stimulated with OVA peptide(Loh15) in the presece of irradiated splenocytes. Cells were cultured in the presence of rTGFbeta rmIL-21,rmIL-6, IL-4 mAb and IFNg mAb for 7 days. Cells were stimulated with anti-TCRbeta mAb for 24hrs. RSM01170 B6 c-kit+ PEC DT-/stimu- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured by RPMI1640 medium RSM01171 BaS TRECK c-kit+ PEC DT-/stimu- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured by RPMI1640 medium RSM01172 MaS TRECK c-kit+ PEC DT-/stimu- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured by RPMI1640 medium RSM01173 B6 c-kit+ PEC DT+/stimu- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT(300ng/ml) and washx1 and 24h cultured by RPMI1640 medium RSM01174 BaS TRECK c-kit+ PEC DT+/stimu- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT(300ng/ml) and washx1 and 24h cultured by RPMI1640 medium RSM01175 MaS TRECK c-kit+ PEC DT+/stimu- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT(300ng/ml) and washx1 and 24h cultured by RPMI1640 medium RSM01176 B6 c-kit+ PEC DT-/stimu a-IgE isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01177 BaS TRECK c-kit+ PEC DT-/stimu a-IgE isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01178 MaS TRECK c-kit+ PEC DT-/stimu a-IgE isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01179 B6 c-kit+ PEC DT+/stimu a-IgE isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01180 BaS TRECK c-kit+ PEC DT+/stimu a-IgE isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01181 MaS TRECK c-kit+ PEC DT+/stimu a-IgE isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01182 B6 DX5+ BM DT-/ stimu- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured by RPMI1640 medium RSM01183 BaS TRECK DX5+ BM DT-/stimu- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured by RPMI1640 medium RSM01184 MaS TRECK DX5+ BM DT-/ stimu- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured by RPMI1640 medium RSM01185 B6 DX5+ BM DT+/ stimu- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by purified by DX5 beads from BM, and 12h cultured with DT(300ng/ml) and washx1 and 24h cultured by RPMI1640 medium RSM01186 BaS TRECK DX5+ BM DT+/ stimu- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by purified by DX5 beads from BM, and 12h cultured with DT(300ng/ml) and washx1 and 24h cultured by RPMI1640 medium RSM01187 MaS TRECK DX5+ BM DT+/ stimu- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by purified by DX5 beads from BM, and 12h cultured with DT(300ng/ml) and washx1 and 24h cultured by RPMI1640 medium RSM01188 B6 DX5+ BM DT-/stimu a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by purified by DX5 beads from BM, and 12h cultured and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01189 BaS TRECK DX5+ BM DT-/stimu a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by purified by DX5 beads from BM, and 12h cultured and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01190 MaS TRECK DX5+ BM DT-/stimu a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by purified by DX5 beads from BM, and 12h cultured and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01191 B6 DX5+ BM DT+/stimu a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01192 BaS TRECK DX5+ BM DT+/stimu a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01193 MaS TRECK DX5+ BM DT+/stimu a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by anti-c-kit biotin Ab and avidin beads from peritoneal cavity, and 12h cultured with DT and washx1 and 24h cultured with a-IgE by RPMI1640 medium RSM01194 B6 DX5+ BM DT-/ stimu IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by DX5 beads from BM, and 12h cultured and washx1 and 24h cultured with rIL-3 by RPMI1640 medium RSM01195 BaS TRECK DX5+ BM DT-/stimu IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by DX5 beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured with IL-3 by RPMI1640 medium RSM01196 MaS TRECK DX5+ BM DT-/ stimu IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by DX5 beads from peritoneal cavity, and 12h cultured and washx1 and 24h cultured with IL-3 by RPMI1640 medium RSM01197 B6 DX5+ BM DT+/ stimu IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by DX5 beads from BM, and 12h cultured with DT and washx1 and 24h cultured with rIL-3 by RPMI1640 medium RSM01198 BaS TRECK DX5+ BM DT+/stimu IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by DX5 beads from peritoneal cavity, and 12h cultured with DT and washx1 and 24h cultured with rIL-3 by RPMI1640 medium RSM01199 MaS TRECK DX5+ BM DT+/ stimu IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a female adult healthy purified by DX5 beads from peritoneal cavity, and 12h cultured with DT and washx1 and 24h cultured with rIL-3 by RPMI1640 medium RSM01200 B6 CD4T cesll isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J 6 weeks female adult healthy CD4T cells were isolated with CD4 magnetic beads from spleen of B6 mice. Cells were stimulated with anti-TCR and anti-CD28 Ab for 24hrs. RSM01201 SC3/K5 CD4T #227 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J 6 months male adult Psoriasis and similar disorders CD4T cells were isolated with CD4 magnetic beads from spleen of Socs3 flox/flox X K5-Cre Tg mice. Cells were stimulated with anti-TCR and anti-CD28 Ab for 24hrs. RSM01202 SC3/K5 CD4T #79 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J 6 months female adult Psoriasis and similar disorders CD4T cells were isolated with CD4 magnetic beads from spleen of Socs3 flox/flox X K5-Cre Tg mice mice. Cells were stimulated with anti-TCR and anti-CD28 Ab for 24hrs. RSM01229 Th2 cells (mock) cultured T-helper 2 cell spleen Mus musculus C57BL/6J 7 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against Luciferase were transduced into the purified CD4 T cells, and GFP+ cells were sorted by FACSvantage. RSM01230 Th2 cells (E4bp4) cultured T-helper 2 cell spleen Mus musculus C57BL/6J 7 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against E4bp4 were transduced into the purified CD4 T cells, and GFP+ cells were sorted by FACSvantage. RSM01231 B6 non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01232 B6 non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01233 B6 non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01234 B6 non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01235 B6 non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01236 B6 non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01237 B6 non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01238 B6 non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01239 MaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01240 MaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01241 MaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01242 MaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01243 MaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01244 MaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01245 MaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01246 MaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01247 BaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01248 BaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01249 BaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01250 BaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01251 BaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01252 BaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01253 BaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01254 BaS TRECK non tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01255 B6 DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01256 B6 DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01257 B6 DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01258 B6 DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01259 B6 DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01260 B6 DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01261 B6 DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01262 MaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01263 MaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01264 MaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01265 MaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01266 MaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01267 MaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01268 MaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01269 MaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy MaS TRECK Tg RSM01270 BaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01271 BaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01272 BaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01273 BaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01274 BaS TRECK DT 250ng x5 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01275 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01276 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01277 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01278 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01279 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01280 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01281 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01282 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01283 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01284 B6 DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy adult B6 RSM01285 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01286 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01287 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01288 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01289 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01290 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01291 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01292 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01293 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01294 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01295 BaS TRECK DT 500ng x1 tissue blood cell blood Mus musculus C57BL/6J n/a n/a adult healthy BaS TRECK Tg RSM01305 B6 #1 DT- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01306 B6 #2 DT- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01307 B6 #3 DT- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01308 BaS TRECK #2 DT- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01309 BaS TRECK #3　DT- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01310 MaS TRECK #1 DT- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01311 MaS TRECK #3 DT- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01312 B6 #1 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01313 B6 #2 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01314 B6 #3 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01315 BaS TRECK #1 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01316 BaS TRECK #2 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01317 BaS TRECK #3 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01318 MaS TRECK #1 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01319 MaS TRECK #2 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01320 MaS TRECK #3 DT- / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01321 B6 #1 DT+ isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01322 B6 #2 DT+ isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01323 B6 #3 DT+ isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01324 BaS TRECK #2 DT+ isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01325 BaS TRECK #3 DT+ isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01326 MaS TRECK #1 DT+ isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01327 MaS TRECK #3 DT+ isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01328 B6 #1 DT+ / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01329 B6 #2 DT+ / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01330 B6 #3 DT+ / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01331 BaS TRECK #1 DT+/ P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01332 BaS TRECK #2 DT+/ P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01333 BaS TRECK #3 DT+/ P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01334 MaS TRECK #1 DT+ / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01335 MaS TRECK #2 DT+ / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01336 MaS TRECK #3 DT+ / P+I isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01337 B6 #1 DT- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01338 B6 #2 DT- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01339 B6 #3 DT- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01340 BaS TRECK #1 DT- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01341 BaS TRECK #2 DT- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01342 BaS TRECK #3 DT- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01343 MaS TRECK #3 DT- isolated mast cell peritoneal cavity Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01344 B6 #1 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01345 B6 #2 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01346 B6 #3 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01347 BaS TRECK #1 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01348 BaS TRECK #2 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01349 BaS TRECK #3 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01350 MaS TRECK #1 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01351 MaS TRECK #2 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01352 MaS TRECK #3 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01353 B6 #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01354 B6 #2 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01355 B6 #3 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01356 BaS TRECK #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01357 BaS TRECK #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01358 BaS TRECK #2 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01359 MaS TRECK #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01360 MaS TRECK #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01361 MaS TRECK #2 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01362 B6 #1 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01363 B6 #2 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01364 B6 #3 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01365 BaS TRECK #1 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01366 BaS TRECK #1 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01367 BaS TRECK #1 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01368 MaS TRECK #1 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01369 B6 #1 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01370 B6 #2 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01371 B6 #2 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01372 BaS TRECK #1 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01373 BaS TRECK #2 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01374 BaS TRECK #3 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01375 MaS TRECK #1 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01376 MaS TRECK #2 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01377 MaS TRECK #3 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01378 B6 #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01379 B6 #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01380 B6 #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01381 BaS TRECK #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01382 BaS TRECK #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01383 BaS TRECK #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01384 MaS TRECK #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01385 MaS TRECK #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01386 MaS TRECK #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01434 B6 #2 DT- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01435 B6 #3 DT- isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01436 B6 #1 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01437 B6 #2 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01438 B6 #3 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01439 MaS TRECK #1 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01440 MaS TRECK #2 DT- /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01441 B6 #2 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01442 B6 #3 DT+ isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01443 B6 #1 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01444 B6 #2 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01445 B6 #2 DT+ /a-IgE isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01450 CD4cre#1 isolated CD4-positive, alpha-beta T cell lymph node Mus musculus C57BL/6 12 weeks female adult immune mediated disease CD4 T cells were enriched by MACS from EAE-induced mice (CD4cre).Cells were stimulated with MOG peptide in the presence of APC for 48hrs. RSM01451 CD4cre#2 isolated CD4-positive, alpha-beta T cell lymph node Mus musculus C57BL/6 12 weeks female adult immune mediated disease CD4 T cells were enriched by MACS from EAE-induced mice (CD4cre).Cells were stimulated with MOG peptide in the presence of APC for 48hrs. RSM01452 CD4cre#3 isolated CD4-positive, alpha-beta T cell lymph node Mus musculus C57BL/6 12 weeks female adult immune mediated disease CD4 T cells were enriched by MACS from EAE-induced mice (CD4cre).Cells were stimulated with MOG peptide in the presence of APC for 48hrs. RSM01453 Socs1f/f CD4cre#1 isolated CD4-positive, alpha-beta T cell lymph node Mus musculus C57BL/6 12 weeks female adult immune mediated disease CD4 T cells were enriched by MACS from EAE-induced mice (Socs1f/f CD4cre).Cells were stimulated with MOG peptide in the presence of APC for 48hrs. RSM01454 Socs1f/f CD4cre#2 isolated CD4-positive, alpha-beta T cell lymph node Mus musculus C57BL/6 12 weeks female adult immune mediated disease CD4 T cells were enriched by MACS from EAE-induced mice (Socs1f/f CD4cre).Cells were stimulated with MOG peptide in the presence of APC for 48hrs. RSM01455 Socs1f/f CD4cre#3 isolated CD4-positive, alpha-beta T cell lymph node Mus musculus C57BL/6 12 weeks female adult immune mediated disease CD4 T cells were enriched by MACS from EAE-induced mice (Socs1f/f CD4cre).Cells were stimulated with MOG peptide in the presence of APC for 48hrs. RSM01487 CD4Cre no stim isolated T cell spleen Mus musculus C57BL/6 n/a n/a adult healthy CD4+ spl. of CD4 Cre Tg were cultured 24h. RSM01488 CD4Cre /a-TCR + PV-1 isolated T cell spleen Mus musculus C57BL/6 n/a n/a adult healthy CD4+ spl. of CD4 Cre Tg were cultured 24h. RSM01489 CBF-beta(F/F) CD4 Cre no stim. isolated T cell spleen Mus musculus C57BL/6 n/a n/a adult healthy CD4+ spl. of CD4 Cre Tg were cultured 24h. RSM01490 CBF-beta(F/F) CD4 Cre /a-TCR + PV-1 isolated T cell spleen Mus musculus C57BL/6 n/a n/a adult healthy CD4+ spl. of CD4 Cre Tg were cultured 24h. RSM01491 B6 Th1 R3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6J n/a n/a adult n/a B6 Th1 R3 RSM01497 B6 #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01498 B6 #2 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01499 B6 #3 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01500 MaS TRECK #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01501 MaS TRECK #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01502 MaS TRECK #1 DT- / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01503 B6 #1 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01504 B6 #2 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01505 B6 #3 DT+ / IL-3 isolated mature basophil bone marrow Mus musculus C57BL/6J n/a n/a adult healthy 24h culture RSM01506 Th2 cells (mock)1 cultured T-helper 2 cell spleen Mus musculus C57BL/6 7 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against Luciferase were transduced into the purified CD4 T cells, and GFP+ cells were sorted by FACSvantage.GFP+ cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01507 Th2 cells (E4bp4)1 cultured T-helper 2 cell spleen Mus musculus C57BL/6 7 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against E4bp4 were transduced into the purified CD4 T cells, and GFP+ cells were sorted by FACSvantage.GFP+ cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01508 Th2 cells (mock)2 cultured T-helper 2 cell spleen Mus musculus C57BL/6 7 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against Luciferase were transduced into the purified CD4 T cells, and GFP+ cells were sorted by FACSvantage.GFP+ cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01509 Th2 cells (E4bp4)2 cultured T-helper 2 cell spleen Mus musculus C57BL/6 7 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against E4bp4 were transduced into the purified CD4 T cells, and GFP+ cells were sorted by FACSvantage. GFP+ cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01510 B6WT CD4T isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6 8 weeks female adult healthy CD4 T cells were enriched by MACS from B6WT mice and stimulated with anti-TCR and anti-CD28 mAbs for 48hrs.Culture supernatant was harvested. RSM01511 LM CD4T isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6 8 weeks female adult healthy CD4 T cells were enriched by MACS from LM mice and stimulated with anti-TCR and anti-CD28 mAbs for 48hrs.Culture supernatant was harvested. RSM01512 E4BP4Tg CD4T isolated CD4-positive, alpha-beta T cell spleen Mus musculus C57BL/6 8 weeks female adult healthy CD4 T cells were enriched by MACS from E4BP4Tg mice and stimulated with anti-TCR and anti-CD28 mAbs for 48hrs.Culture supernatant was harvested. RSM01521 DO11.10 no stim isolated T cell thymus Mus musculus BALB/c n/a female adult healthy thymus CD4MP 36h culture RSM01522 DO11.10 a-TCR + PV-1 isolated T cell thymus Mus musculus BALB/c n/a female adult healthy thymus CD4MP 36h culture RSM01523 OT-2 Tg no stim isolated T cell thymus Mus musculus C57BL/6J n/a female adult healthy thymus CD4MP 36h culture RSM01524 OT-2 Tg a-TCR + PV-1 isolated T cell thymus Mus musculus C57BL/6J n/a female adult healthy thymus CD4MP 36h culture RSM01610 Foxp3+ CD4T(spl) isolated CD4-positive, CD25-positive, alpha-beta regulatory spleen Mus musculus C57BL/6 8 weeks male adult healthy Foxp3(hCD2)+ CD4T cells were enriched with MACS from spleen of Foxp3/hCD2 mice and were stimulated with anti-TCRAb(H57) and anti-CD28Ab(PV-1) for 48hrs. Culture supernatant was harvested. RSM01611 Foxp3- CD4T cells(spl) isolated CD4-positive, CD25-positive, alpha-beta regulatory spleen Mus musculus C57BL/6 8 weeks male adult healthy Foxp3(hCD2)- CD4T cells were enriched with MACS from spleen of Foxp3/hCD2 mice and were stimulated with anti-TCRAb(H57) and anti-CD28Ab(PV-1) for 48hrs. Culture supernatant was harvested. RSM01612 Foxp3+ CD4T(thy) isolated CD4-positive, CD25-positive, alpha-beta regulatory thymus Mus musculus C57BL/6 8 weeks male infant healthy Foxp3(hCD2)+ CD4T cells were enriched with MACS from thymus of Foxp3/hCD2 mice and were stimulated with anti-TCRAb(H57) and anti-CD28Ab(PV-1) for 48hrs. Culture supernatant was harvested. RSM01613 Foxp3- CD4T cells(Thy) isolated CD4-positive, CD25-positive, alpha-beta regulatory thymus Mus musculus C57BL/6 8 weeks male infant healthy Foxp3(hCD2)- CD4T cells were enriched with MACS from thymus of Foxp3/hCD2 mice and were stimulated with anti-TCRAb(H57) and anti-CD28Ab(PV-1) for 48hrs. Culture supernatant was harvested. RSM01614 Th2 cells (n.c*n.c) cultured T-helper 2 cell spleen Mus musculus C57BL/6 8 weeks female infant healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against Luciferase were transduced into the purified CD4 T cells, and transduced cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01615 Th2 cells (Nfil3*n.c) cultured T-helper 2 cell spleen Mus musculus C57BL/6 8 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against Nfil3 were transduced into the purified CD4 T cells, and transduced cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01616 Th2 cells (n.c*gata3) cultured T-helper 2 cell spleen Mus musculus C57BL/6 8 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against gata3 were transduced into the purified CD4 T cells, and transduced cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01617 Th2 cells (Nfil3*gata3) cultured T-helper 2 cell spleen Mus musculus C57BL/6 8 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against Nfil3 and gata3 were transduced into the purified CD4 T cells, and transduced cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01618 Th2 cells (n.c) cultured T-helper 2 cell spleen Mus musculus C57BL/6 8 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against Luciferase were transduced into the purified CD4 T cells, and transduced cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01619 Th2 cells (Nfil3) cultured T-helper 2 cell spleen Mus musculus C57BL/6 8 weeks female adult healthy CD4 T cells were enriched by MACS and stimulated with anti-TCR and anti-CD28 mAbs in the presence of mrIL-4 and anti-IFNg Ab for 1 week. The viral particles containing shRNA against Nfil3 were transduced into the purified CD4 T cells, and transduced cells were restimulated with anti-TCR for 24hrs.Culture supernatant was harvested. RSM01675 SKO +/+ CD4+spl. #1 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01676 SKO +/+ CD4+spl. #2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01677 SKO +/+ CD4+spl. #3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01678 SKO +/+ CD4+spl. #4 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01679 SKO -/- CD4+spl. #1 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO(-/-) F2 RSM01680 SKO -/- CD4+spl. #2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO(-/-) F2 RSM01681 SKO -/- CD4+spl. #3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO(-/-) F2 RSM01682 SKO -/- CD4+spl. #4 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO(-/-) F2 RSM01683 SKO +/+ CD4+CD62L^high spl. #1 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01684 SKO +/+ CD4+CD62L^high spl. #2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01685 SKO +/+ CD4+CD62L^high spl. #3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01686 SKO -/- CD4+CD62L^high spl. #1 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO(-/-)F2 RSM01687 SKO -/- CD4+CD62L^high spl. #2 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01688 SKO -/- CD4+CD62L^high spl. #3 isolated CD4-positive, alpha-beta T cell spleen Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01689 SKO +/+ DX5+ BM #1 isolated mature basophil bone marrow Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01690 SKO +/+ DX5+ BM #2 isolated mature basophil bone marrow Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01691 SKO +/+ DX5+ BM #3 isolated mature basophil bone marrow Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01692 SKO -/- DX5+ BM #1 isolated mature basophil bone marrow Mus musculus hybrid n/a n/a adult healthy SKO(-/-) B6 back F2 RSM01693 SKO -/- DX5+ BM #2 isolated mature basophil bone marrow Mus musculus hybrid n/a n/a adult healthy SKO(-/-) B6 back F2 RSM01694 SKO -/- DX5+ BM #3 isolated mature basophil bone marrow Mus musculus hybrid n/a n/a adult healthy SKO(-/-) B6 back F2 RSM01695 SKO +/+ whole BM #1 isolated blood cell bone marrow Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01696 SKO +/+ whole BM #2 isolated blood cell bone marrow Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01697 SKO -/- whole BM #1 isolated blood cell bone marrow Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2 RSM01698 SKO -/- whole BM #2 isolated blood cell bone marrow Mus musculus hybrid n/a n/a adult healthy SKO Wild type F2